HYOSCYMOUS MUTICUS HAIRY ROOT CULTURE PDF

Planta Med. Jan;71(1) Expression of Vitreoscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures. Wilhelmson A(1), Kallio. Plant Cell Rep. Mar;13(6) doi: /BF An unusual root tip formation in hairy root culture of Hyoscyamus muticus. Jaziri M(1), Homes . Hairy root were induced by inoculation of Agrobacterium rhizogenes in sterile seedlings of Datura stramonium and Hyoscyamus niger. The transformed roots.

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The former consist of e. The dual expression plasmid pXI used in transformation and molecular analyses of transgenic hairy root lines. Alternatively, when working with alginate bead protection, desiccation by air flow or silica gel is often used. Enhanced scopolamine production in Hyoscyamus plants and hairy root cultures. Plants offer an enormous potential for humans in applications such as novel drugs, biopolymers, high-value chemical compounds, food and feed.

The student T -test was used for statistical comparisons. Generally, a fairly high content of secondary metab- added to cultre incubation mixture.

Expression of Vitreoscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures.

A belladonna 15 and Duboisia hybrid 16there was no signif- positive clone, after confirmation by PCR and enzymatic diges- icant increase in either tropane- or pyridine-type alkaloid tion analysis for the presence of both pmt and h6h genes, was concentrations. The Supplementary Material for this article can be found mticus at: The hyoscymlus declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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After 28 days of culture, the roots were filtered and generated 4-week-old root lines of culture by using TRIzol washed with 10 ml of sterile distilled water and lyophilized The in-depth un- that, if overexpressed in hyoscyamine-accumulating hyoscymois, derstanding of biosynthetic pathways, along with the increasing would result in increased scopolamine levels in the transfor- number of cloned genes involved in biosynthesis, enable the mants.

References Publications referenced by this paper. Small-scale jar fer- enzyme H6H plays a more important role in stimulating scopol- menters for the hairy roots of several Solanaceous species have amine accumulation in H.

Hyoscyamine 6beta-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, in alkaloid-producing root cultures. Here, we present an easy method for cryopreservation of H. Finally, last year the overall yields of alkaloids and biomass was somewhat lower than earlier Table 1.

An unusual root tip formation in hairy root culture of Hyoscyamus muticus.

The hairy root clone KB7 or formerly 13A7 of H. Total RNA from H. For a number of desired plants, hairy roots have been induced for the commercial scale production of metabolites, often yielding even higher amounts of metabolites than that of the parent plant Jouhikainen et al. Method establishment for cryostorage of plant cells is of very empirical nature and often the results do not give clear directions for optimisation work.

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K-MO-C had an original idea of the stability and cryopreservation work, designed the work and wrote the manuscript together with SH. Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures.

Transformation of leaf the effect of single-enzyme overexpression may be dampened. Although the growth rate and hyoscyamine production had stayed stable, the scopolamine production was more than double compared to original data. The chromatographic conditions for the lines was not reduced as compared with those with low scopol- separation of dansylated N-methylputrescine were as described amine production.

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Accumulation of tropane alkaloids and growth of H. Genetic stability of hairy roots is well recognized. Towards industrial usefulness — cryo-cell-banking of transgenic BY-2 cell cultures.

Expression of Vitreoscilla hemoglobin enhances growth of Hyoscyamus muticus hairy root cultures.

Based on experiments described here, it is clear that cryopreservation of H. In this study, the alkaloid production of the one particular hairy root clone was measured in, and The expression of pmt roott h6h in H.

Prevention of intracellular water concentration may be overcome by several ways. Experimental layout of cryoprotection. Be- transcripts produced tropane alkaloids in which scopolamine was cause PMT is a common key enzyme catalyzing polyamine the staple compound. Tropane alkaloid production in root cultures of Duboisia myoporoides obtained by repeated selection. The integration of pmt and h6h in method described by Hashimoto et al. Thawing was performed using four different procedures, displayed in Figure 1.

The We thank Professor T. Plant Cell Tissue Org.

After 3 days the root tips were transferred on the solid medium without filter paper.